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I know there is an interest in biohacking alternative testing methods around these parts so I figured I would make a thread for anyone who may want to do research at home.

What you will need to get started:
An incubator
A microscope 
Growth media
Something to sterilize your growth media
Plates
Microscope slides and covers
Staining kits
Immersion oil

For the incubator you can buy one new on Amazon or used on ebay or you could even make your own.  They are not difficult to build and there are plenty of resources online. 

If you want to culture bacteria you are going to need a microscope with 1000x magnification power and for that you are going to need a 100x oil immersion lense.  You need to buy it new and you need to treat it with care.  The lenses scratch easily and must be cleaned with Kim wipes.  If you are only going to be working with fungi then you only need to go up to a 400x lense.

For growth media you are going to want to use agar agar in most cases.  You can find it as an vegetarian thickener in organic grocery stores or you can buy it on Amazon.  If you aren't buying lab grade you will need to sterilize your media before using it.  You can also use guar gum as an alternative to agar but it is not as firm as agar and may require additional thickeners like starch.  Be warned that guar gum can be eaten by some microbes and starch can be eaten by many microbes so for more controlled expirements you should use agar.

To sterilize your media an autoclave works best, but those are expensive and a pressure cooker will work to sterilize your equipment in most cases and in a pinch you can even microwave, but don't expect the same results as an autoclave.

For your plates you can easily buy them on Amazon or you can use polypropylene containers from the grocery store.  They have to be polypropylene because they need to survive the autoclave.

Slides and covers can be purchased on Amazon or cannibalized form a child's microscope kit at Walmart if you need them in a hurry.

Staining kits will most likely need to be purchased online.  The only two staining reagents that can be found in stores are methylene blue (pharmacy) and malachite green (aquarium supply). For bacteria you will find gram stains, acid fast stains, and capsule stains will get you pretty far, but there are all kinds of staining techniques you can use in your projects.

It is best to buy immersion oil online, but anis oil, clove oil, and ceder wood oil will all work in a pinch.  Be sure to clean you lense every time especially if you choose to use an over the counter alternative.  You will ruin your lense if you don't clean it.

Resources
https://microbewiki.kenyon.edu/index.php/MicrobeWiki
https://www.the-odin.com/
https://diybio.org/

Have fun expirementing and please don't do anything stupid.
I'm going to throw out some project ideas that could be useful to our communities and kind of set the tone for the thread here so that this doesn't become "The Anarchists cookbook: biology addition"

1. Mycomaterials
Mycelium can be used to produce everything from vegetarian leather to building materials to meat alternatives to medicines.  It's cheap to produce and very environmentally friendly.  Having first mover advantage in this field would be very useful for us.

2. Microbial fertilizers
Many microbes can be used to enhance crop yield similar to how probiotics can be used to improve health.  A completely organic alternative to industrial fertilizers.  Theoretically these could be even better than conventional fertilizers but no one has obtained yields better than 20% of what conventional fertilizers offer.  Be aware this project will be crop and site specific.  This will not be an easy project and it will not be a one person project.

3.  Probiotic, prebiotic, and postbiotic supplements
Your gut microbiome is very important for your health.  Supplements that aim to improve the gut microbiome will go a long away in preventing illnesses.  I personally would like to see something that increases the population of clostridium sporogenes in the gut as it is known to prevent dementia and certain types of cancers.
Replies: >>70 >>71
>>69
Do you have any other cool /sci/ stuff for me anon?
Replies: >>72
>>69
4.  Cheap, eco-friendly, and easily accessible alternatives to common staining reagents.
There is research in this area so we wouldn't be starting from scratch here.  Many staining reagents are expensive and hazardous those of us wanting to do expirements in a home setting could really use alternatives and this is a project people could easily contribute to by trying different stains at home and posting results.

5. Standardized small scale bioreactor designs.
Microbes can produce all kinds of things from solvents to medicines to fuels to nanoparticles.  Having standardized bioreactor designs for species of interest would help anyone wanting to do this kind of research at home or even producing products.
>>70
What are you looking for?
Replies: >>73 >>74
>>72
Whatever
Replies: >>75
>>72
Whatever you have
Replies: >>75
>>73
>>74
I'll post a thread on 3D printing later.  For now I will leave you with an unfinished DIY atomic force microscope.  (Not my project, but an AF microscope for under $2k could be extremely useful both biology projects and material science projects.)  
https://hackaday.io/project/191403-atomic-force-microscope-from-ground-up
Replies: >>76
>>75

Do you do any electrical stuff?
Have you done any of this diybio yet? Did it work and what results?
Replies: >>77
>>76
I don't do much with electronics but I do have my own microscope and incubator.  I have played around with guar gum as an alternative to agar and it worked, but it wasn't very good because it was so goopey.  I have also played with mycelium bricks using cardboard as a feed stock.  I was actually impressed with the results there.  They had similar properties to balsa wood, but they need to be weatherproofed.  I made a design for an incubation chamber for mycelium leather, but it was obsolete before I even got it off the computer.  An other researcher made a gel culture that could do what I was wanting to do with my machine for a much lower price tag.  I could post the design later if anyone wants to pick it up.  It could be repurposed for growing specialty mushrooms.
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Hey. So what I want to be able to do is test supplements and peptides etc for purity. I am also interested in the legal chemical synthesis of racetams, and maybe indoor mass industrial production of supplements using hydroponics or something. The first question is just a sufficiently powered microscope, isn't it?

Alibaba has some interesting industrial equipment when it comes to manufacturing. Cheap too, a wagie with savings could easily set up a shop floor factory doing this
Replies: >>79 >>111 >>112
>>78
If you are testing for bacterial contamination you will want a microscope with 1000x magnification and if you are testing for chemical contaminations you are going to need to use various types of spectroscopy.  You should look into the ASTMs (American Standard Test Methods) for peptide testing.  I'm sure something exists.  I know FTIR (Fourier Transformation Infrared) spectroscopy will be one of the methods you will use but I don't know how sensitive your machine will need to be and I don't know what other tests you will need to run.  Used FTIR machines can be affordable.  NMR (Nuclear Magnetic Resonance), GC (Gas Chromatography), and HPLC (High Performance Liquid Chromatography) would all be nice too but those machines can be expensive even if used.  UV spectroscopy could also be useful and those machines are usually affordable if used.  Ebay is a great source of used lab equipment as are universities.  Expect many universities to be shutting down over the next 10 years.  There will be good opportunities to find used lab equipment in the near future with the enrollment crisis.

I'm glad someone wants to pick that project up.  I hate analytical chemistry.  It can be very tedious.  Also if you end up working with other people be ready to tell them when they are fucking up and be ready to run tests again and troubleshoot machines.
Replies: >>81
>>79
Another great source of used scientific equipment is failing laboratories trying to sell off their stock.

Also a warning.  If the government ever decides they want to shut down /ourlabs/ the EPA, FDA, and OSHA will make more effective attack vectors than someone like shitwood could ever hope to be.  A single OSHA fine for a chemical without a lid on it is $50000 which is enough to bankrupt a small businesse starting out.  Make sure you have higher standards than the EPA, the FDA or OSHA require as you will not be held to the same standards as Mr.  Usurywitz.  Frankly we should have higher standards for ourselves anyways especially if we want people to trust our services.
>>84
For fun scientific purposes it is fine.  Just make sure you dispose of your waste properly and don't grow any organisms above a level one.
>>84
Pretty sure salmonellla is a level 3.  There might be some level 1 strains used in cancer research though.  I know salmonella is being investigated for all kinds of cancer treatments.  Check microbe wiki for culturing instructions.
Replies: >>87
>>86
https://pmc.ncbi.nlm.nih.gov/articles/PMC9609134/
I will leave these here as they are useful and easy to use tools.
https://en.m.wikipedia.org/wiki/Directed_evolution
https://en.m.wikipedia.org/wiki/Mutation_breeding
>>84
This is an essential technique for anyone learning microbiology.
https://byjus.com/neet/streak-plate-technique/#:~:text=Streak%20Plate%20Method%20Principle,-The%20streak%20plate&text=In%20this%20technique%2C%20a%20loopful,colony%20forming%20units%20(CFUs).
>>78
So I looked for what was needed for peptide purification analysis and you are going to need a reverse phase high performance liquid chromatography (RP-HPLC) machine with UV absorption atat 220 nm.  Most HPLC machines will run reverse phase in adition to normal but you will have to buy a special column that will probably run you around $200-500.  You can expect to spend $3000 minimum on a used HPLC machine.  Make sure it can do what you need it to do.
Replies: >>112 >>120
>>78
>>111

Realistically you are looking closer to $5000-7000 dollars for that machine used.  If you are new to spectroscopy I recommend using FTIR and UV spectroscopy to determine the purity of herbal extracts.  Those machines are much more affordable and after you have some experience and results to show our community people might be interested in crowd sourcing an HPLC machine for you.  If you can show good results with FTIR and UV spectroscopy I would be willing to throw a couple hundred towards the HPLC machine.
Replies: >>120
>>112
>>111
what kind of lab training would I need to run this? Is it online? Consider I have no stem background
Replies: >>121 >>123
>>120
There are plenty of videos online and the machines usually have a user manual you can find online.
Replies: >>122
>>121
Oh I forgot to mention that some of these companies have software that they like to force you to buy a license for.  So make sure you research your machine before purchasing it.
>>120
Also it would be a good idea to learn chemistry from the basics all the way to organic, bio, and analytical chemistry.  Quantum chemistry would be helpful if you plan on making serious modifications to the equipment or designing your own test methods but it is not necessary if you simply want to run basic tests.
>>142
The Odin website I linked has a kit with everything you need to try.
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